RKO-CAS9 cell line

location： Home > Products > Cas9 Stable Cell Lines > RKO-CAS9

RKO-CAS9 Order

Catalog#

YC-C002-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

RKO-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the RKO cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a RKO-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
RKO-CAS9	Cas9	21.27658653	3.43598557
RKO-CAS9	β-actin	17.84060097	3.43598557
RKO	Cas9	35.33678818	17.99855232
RKO	β-actin	17.33823586	17.99855232

Cutting Efficiency Validation

RKO-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the RKO-CAS9 stable cell pool which is electroporated by TP53 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:RKO			STR Info (Cell bank) Cell Line:p53R [JHU-56]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	13
D13S317	8	11		8	11
D7S820	8	10		8	10
D16S539	13	13		12	13
VWA	16	22		16	22
TH01	6	10		6	10
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	8	10		8	10
D12S391	15	19	20.0
FGA	20	21	23.0
D2S1338	16	16
D21S11	27	29	30.0
D18S51	11	12
D8S1179	9	13	14.0
D3S1358	16	19
D6S1043	14	19
PENTAE	11	13
D19S433	14	14
PENTAD	10	11
D1S1656	13	14	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

RKO-CAS9

Catalog#

YC-C002-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

KO Cell Lines

KRAS Knockout cell line(RKO)
inquiry More details

Organism: Human

Gene Name: KRAS

Gene ID: 3845

Size: 1*10^6

Catalog#: YKO-H251

YKO-H251(RKO)
KREMEN1 Knockout cell line (RKO)
inquiry More details

Organism: Human

Gene Name: KREMEN1

Gene ID: 83999

Size: 1*10^6

Catalog#: YKO-H004

YKO-H004(RKO)

EGFP Stable Cell Lines

RKO-EGFP
inquiry More details

Catalog#: YC-C002-EGFP-P

Size: 1*10^6

Instruction:

Luciferase Stable Cell Lines

RKO-Luc
inquiry More details

Catalog#: YC-C002-Luc-P

Size: 1*10^6

Instruction:

Wild-type Cell Lines

Human Colon Adenocarcinoma Cell Line(RKO)
inquiry More details

Catalog#: YC-C002

Size: 1*10^6

Instruction:

Related Popular Products

KO Cell Lines

KRAS Knockout cell line(RKO)

Organism: Human

Gene Name: KRAS

Gene ID: 3845

Size: 1*10^6

Catalog#: YKO-H251

inquiry More details
KREMEN1 Knockout cell line (RKO)

Organism: Human

Gene Name: KREMEN1

Gene ID: 83999

Size: 1*10^6

Catalog#: YKO-H004

inquiry More details

EGFP Stable Cell Lines

RKO-EGFP

Price (USD): -

Catalog#: YC-C002-EGFP-P

Size: 1*10^6

Instruction:

inquiry More details

Luciferase Stable Cell Lines

RKO-Luc

Price (USD): -

Catalog#: YC-C002-Luc-P

Size: 1*10^6

Instruction:

inquiry More details

Wild-type Cell Lines

Human Colon Adenocarcinoma Cell Line(RKO)

Price (USD): -

Catalog#: YC-C002

Size: 1*10^6

Instruction:

inquiry More details

Subscribe Us

* E-mail：

Country/Region：

Name：

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.

RKO-CAS9 cell line

Ubigene